Primerquest | Tool name | Primerquest |
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| URL | http://eu.idtdna.com/Scitools/Applications/Primerquest/Default.aspx |
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| Important features | 1. Designs primer for real time PCR and Junction primers.
2. Designs primers/probes for SYBR green and TaqMan. |
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| Citations | |
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| Year of publication | Not available |
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| Rank by usage frequency | 98 |
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| Comments | |
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| Function | Junction primers, Real time PCR, General PCR, Primer specificity checking |
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| Category | Online, Free |
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| Features | 1. Designs primers for real time PCR and Junction primers.
2. Allows SNP checking, primer pair specificity against the transcriptome, selection of GC clamp and region specificity for primer picking.
3. Designs primers for SYBR green and TaqMan.
4. Option to set the primer parameters such as product length, Tm, GC%, product Tm, etc.
5. Designs primers for exon-exon junctions.
6. Lists the best primers, user can select primers from the list and can export the selected list. |
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| Description | |
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